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Fig. 3 Pak1 nuclear import is contingent upon LC8 dimerization and is dynein independent.
(A) Confocal microscopy images of MCF-7 cells transiently transfected with either the FKBP vector, Pak1 226–249, or Pak1 226–545 show either GFP-Pak1 construct is cytoplasmic in the absence of AP20187 and stained with DAPI to visualize nuclei. Scalebar shown is 10 microns. (B) Quantification of confocal images indicates an ∼5 fold increase in nuclear GFP over non-treated cells upon AP20187 treatment. Data quantified in the same manner as in Fig. 2B.
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