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Fig. 6 Loss of olig2 gene function affects the expression of sim1. A,B: Embryos injected with olig2 MO were subjected to whole mount in situ hybridization with antisense sim1 RNA probe (purple), followed by immuno-staining with anti-TH antibody to detect DA neurons (brown). C,D: Embryos injected with sim1 MO were subjected to whole mount in situ hybridization with olig2 RNA probe (purple). Black arrowhead marks deficient Sim1 expression domain in the PT. All images are presented in lateral view, anterior to the left. NPO, neurosecretory preoptic area; PT, posterior tuberculum; Tel, telencephalon; Tha, thalamus. Scale bar = 50 μm.