Fig. S3
Fig. S3 The effect of Wnt/β-catenin signaling on midline development. (A-P) Midline structures were not affected by alterations of Wnt signaling, as revealed by staining for the midline mesendodermal markers axial (A-H) or gsc and ntl (I-P). Embryos were co-stained with pax2 as an anteroposterior reference. Shown are dorsal views with anterior to the top of tailbud-staged embryos. (Q-T) lefty1 expression in the notochord was abolished in wnt3 morphants (T), but not in apc morphants (R) or in embryos injected with wnt3 RNA (S). Shown are dorsal views of 21-somite staged embryos with anterior to the top.