ZFIN Image: Bonner et al., 2008, Fig. 3

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Figure Caption

Fig. 3 Tcf7 is required for dorsal progenitor patterning. (A) At 15 hpf, tcf7 is expressed widely throughout the neural keel (dotted line), with a dorsal bias. (B) At 24 hpf tcf7 is expressed in the dorsal spinal cord as well as the median fin fold. (C) There is no significant decrease in the mitotic index of the dorsal and intermediate domains in tcf7 morphants, but mitosis in the ventral domain is significantly reduced. *p < 0.05 by T-test, error bars indicate ± SEM. (D, E) Expression of wnt1 and (F, G) radar mRNA is unaffected in tcf7 morphants. (H, I) Expression of msxc and (J, K) pax3 is reduced in tcf7 morphants. (L, M) Expression of dbx2 shifts into the dorsal domain in tcf7 morphants. (N, O) Ventral expression of nkx6.2 is unaffected in tcf7 morphants. (A, B) 12 μm cross-sections are shown. (D–O) Lateral mounts are shown. Scale bars = 10 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 313(1), Bonner, J., Gribble, S.L., Veien, E.S., Nikolaus, O.B., Weidinger, G., and Dorsky, R.I., Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling, 398-407, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.