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Fig. 3 Areas of co-expression between nr4a2a/b, pitx3 and lmx1b.1 in the posterior tuberculum. (A-D″) Confocal z-projections of double whole mount FISH at 48 hpf show overlapping expression domains for nr4a2a/b, pitx3 and lmx1b.1. The projections encompass the focal planes through the ventral diencephalon. The antisense probes used for the FISH are indicated on the top of each panel. (A-A″) 28 μm projection; (B-B″) 19 μm projection; (C-C″) 16 μm projection; (D-D″) 13 μm projection. Anterior is to the left. Scale bars: 50 μm. Abbreviation: PT, posterior tuberculum.
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Full text @ BMC Dev. Biol.