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Fig. S3 Global RA Treatments Rescue Hindbrain Segmentation over a Wide Range of Concentrations. Embryos were treated at 4 hpf with DEAB either with or without a low concentration of RA between 0.625–20 nM, fixed at 19 hpf, and pattern was assessed by in situ hybridization for hoxd4a (blue; r6/7 boundary), krox-20 (egr2b, red; r3 and r5), and pax2a (red; midbrain–hindbrain boundary). (A) Wild-type expression. (B) DEAB-treated embryos show loss of r5–r7. (C–F) Concentrations from 0.625–5 nM rescue wild-type patterning to the same extent. (G–H) Higher concentrations produce anterior defects.
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