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Fig. 6 (A–C) CNS phenotype of nls mutants at 4 dpf (A) Mbp expression in wild type spinal cord at 4 dpf. Differentiating oligodendrocytes are located peripherally in the white matter. (B) Mbp expression is absent in the great majority of nls mutant fish. (C) Sox10 expression in wild type spinal cord. Sox10 labels oligodendrocyte precursors in the grey matter (arrow) as well as differentiating oligodendrocytes in the periphery. (D) Sox10 expression appears normal in nls mutants. Note labeling of oligodendrocyte precursors in the grey matter (arrow). (E–H) Effects of timed application of DEAB (D–F) and RA (G) on wild type and nls mutant fish respectively. (E) Effects of DEAB treatment from 11 hpf on mbp expression. (F) Effects of DEAB treatment from 14 hpf on mbp expression. (G) Effects of DEAB treatment from 26 hpf on mbp expression. (H) Mbp expression is restored in the PLL (arrowheads) of nls mutants treated with exogenous RA between 10 and 16 hpf.
Reprinted from Developmental Biology, 297(1), Kazakova, N., Li, H., Mora, A., Jessen, K.R., Mirsky, R., Richardson, W.D., and Smith, H.K., A screen for mutations in zebrafish that affect myelin gene expression in Schwann cells and oligodendrocytes, 1-13, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.