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Fig. 2 Glial differentiation fails in sox10 embryos. (A,B) Double immunofluorescent detection of trunk sensory neurons (Hu, red) and prospective glial cells (GFP, green) in anterior trunk in 5 dpf wild-type (A) and sox10 mutant (B) embryos carrying the sox10:egfp transgene. Although GFP+ cells occupy similar positions in sox10 mutant and wild-type embryos (arrows), the elongated morphology of Schwann cells and the compact clustered arrangement of satellite glial cells in DRGs (arrowheads) of wild-type embryos (A) are absent in sox10 mutants (B). Neurons are often displaced ventrally in sox10 mutant embryos, but sensory neuron number may be approximately normal at this axial position (B). (C,D) In situ hybridisation with mbp reveals absence of differentiated Schwann cells in sox10 embryos (D) compared with wild-type siblings (arrowhead, C). Likewise, oligodendrocyte differentiation (*, C) fails in sox10 mutants (D). Scale bars: 20 μm in A; 50 μm in B.