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Fig. S1 Injection of a splice-blocking morpholino results in the loss of lef1 mRNA transcripts. (A) Diagram of lef1 gene structure indicating the location of the lef1 MO target at the exon 7 splice donor site. Introns are not to scale. (B) RT-PCR analysis demonstrates that injection of the lef1 MO into zebrafish embryos at the one-cell stage results in the production of aberrantly spliced forms at 12 hpf and the loss of lef1 mRNA after 18 hpf.
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