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Fig. 5 Lef1 is required for neuronal differentiation in the posterior hypothalamus. (A,B) Confocal projections through the hypothalamus of whole-mount 36 hpf brains stained for HuC/D, a pan-neuronal marker. Owing to differences in mounting, neurons outside the hypothalamus may not be visible. (C,D) Cross-sections of 36 hpf brains through the plane marked by the lines in A,B. A specific population of Hu-positive neurons (yellow circles and ovals) is absent in the posterior hypothalamus of lef1 morphants (B,D). (E) Hu (green) is co-expressed with Lef1 protein (red) in a subset of posterior neurons at 36 hpf. (F,G) Acetylated tubulin (AT) staining labels specific axons in the posterior hypothalamus (yellow circles) that are reduced in lef1 morphants at 48 hpf. Confocal projections through the ventral side of the hypothalamus are shown, with anterior towards the top.