ZFIN Image: Nissen et al., 2006, Fig. 5

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Figure Caption

Fig. 5 wdr68 acts upstream of the edn1 pathway. A) In situ hybridization (ISH) with dlx2 on 12 somites stage embryos. Wild type and dys/dys mutant animals were indistinguishable. B) ISH on 20 somites stage wild type sibling animal with edn1 probe. C) dys/dys homozygotes display reduced edn1 expression at 20 somites stage. D) ISH on 28 hpf wild type sibling animal with edn1 probe. E) dys/dys homozygotes display reduced edn1 expression at 28 hpf. F) ISH on 41 hpf wild type sibling with bapx1 probe. G) dys/dys homozygotes display reduced bapx1 expression. H, I, J, K, L, M, N, O) Red arrowhead indicates 1^starch expression domain, blue arrowhead indicates 2^ndarch expression domain. H) ISH on 28 hpf wild type sibling with hand2 probe. I) dys/dys homozygotes display reduced 1^starch hand2 expression, red arrowhead. Red dotted outline indicates expression in developing heart. J) dys+wdr68MO animals display reduced hand2 expression in both 1^stand 2^ndarches, red and blue arrowheads, respectively. K) edn1-MO animals lack hand2 expression in both the 1^stand 2^ndarches. L, M, N, O) Red asterisk indicates olfactory expression domain. Black asterisk indicates ear expression domain. L) ISH on 28 hpf wild type sibling with dlx3b probe. M) dys/dys homozygotes display reduced 1^starch dlx3b expression, red arrowhead. N) dys+wdr68MO animals display reduced dlx3b expression in both 1^stand 2^ndarches, red and blue arrowheads, respectively. O) edn1-MO animals lack dlx3b expression in both the 1^stand 2^ndarches. P, Q, R, S) Red underline indicates 1^starch region for sox9a expression. Black underline indicates 2^ndarch region for sox9a expression. P) ISH with sox9a probe on 28 hpf wild type sibling. Q) Reduction of 1^starch sox9a expression without significant effect on 2^ndarch sox9a expression in dys/dys animals. R) Injecting dys animals with wdr68MO caused further reduction of 1^starch sox9a expression without substantial effects on 2^ndarch sox9a expression. S) edn1-MO animals show reduced but still detectable sox9a expression in both the 1^stand 2^ndarches.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.