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Degarelix can be used for anti-mycobacterium therapy. (a) Bacterial burden values in infected zebrafish larvae treated with mock, rifampicin (10 ?M), or degarelix (1 ?M) for 72 h. Data are represented in a scatter dot plot. (b) Adult zebrafish infected with M. marinum (400 CFU) for 1 week were orally treated (daily) with degarelix (10 mg/kg) and RFP (5 mg/kg) as a positive control up to 14 days. Bacterial burdens are shown in (b). (c-e) Histopathology assessments in whole fish sections using H&E or acid-fast staining at 14 dpi (scale bar = 20 ?m). Granulomas were compared between infected adult zebrafish treated with/without degarelix for <10 or >10 bacterial burden scores (d) and necrotic granuloma percentages (e). (f) Bacterial burden values in infected zebrafish larvae treated with mock, rifampicin (10 ?M), degarelix (1 ?M), or degarelix (1 ?M) + RFP(10 ?M) for 72h.
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