dab2 is expressed in BLECs. (a) Schematic representations of a 4dpf zebrafish embryo head from a lateral view. In the top drawing, a blue square marks the region shown magnified in the bottom drawing and in (b). The bottom drawing depicts BLECs (green, arrowheads) that are arranged in loops at the dorsal side of the TeO, at the midbrain-hindbrain boundary and on the ventral side of the hindbrain. Major blood vessels are illustrated in red. (b) Lateral view of an in situ hybridization against dab2 transcripts, highlighting dab2 expression within BLECs in casper-/- embryos at 80hpf (arrowheads). (c) Heat-map representing relative gene expression levels within BLECs (YuGene data published by Bower et al.10) for known lymphatic endothelial markers (lyve1b, prox1a, flt4, mafba, stabilin1, stabilin2, lyve1a, nrp2a, mmp2, mrc1a, ors2, slc7a7), the pan-endothelial marker fli1a and several genes not expressed by lymphatic endothelial cells (flt1, notch3, cdh5, kdrl, pdgfrß). Note the high dab2 expression levels in BLECs. The colour scale indicates relative expression from 1 (highest) to 0 (lowest). BLEC, brain lymphatic endothelial cell; dpf, days post fertilization; hpf, hours post fertilization; MsV, mesencephalic vein; TeO, optic tectum.
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