FIGURE

Figure 2

ID

ZDB-FIG-241028-138

Publication

Li et al., 2024 - Deletion of Slc1a4 Suppresses Single Mauthner Cell Axon Regeneration In Vivo through Growth-Associated Protein 43

Other Figures

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Figure 2

Identification of slc1a4 mutant zebrafish. (a) Schematic of Cas9-sgRNA targeted site located in first exon of slc1a4. (b) Schematic of complex injected into one-cell embryos. (c) Procedure for obtaining slc1a4^−/− mutant lines. (d) Representative sequencing results of wild-type and mutated zebrafish lines. Mutant sequencing results showed that 10 bp were deleted in slc1a4^−/− zebrafish. (e,f) Western blotting analysis showed that Slc1a4 protein expression is inhibited in mutant group compared with wild type. Protein expressions were quantified by Image J software version 1.54f. Experiment was repeated three times with three independent samples. **** p < 0.0001. Assessed by unpaired t-test.

Expression Data

Antibody:	Ab1-slc1a4
Fish:	AB slc1a4^{ah8023/ah8023} (AB)
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc1a4^{ah8023/ah8023} (AB)
Observed In:	whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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