FIGURE

Fig. 1

ID

ZDB-FIG-241002-1

Publication

Lavorato et al., 2024 - dldhcri3 zebrafish exhibited altered mitochondrial ultrastructure, morphology and dysfunction partially rescued by probucol or thiamine

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Fig. 1

DLD role in metabolic pathways, generation of the dldh?/? zebrafish model, and confirmation of Dldh protein deficiency in dldh?/? zebrafish larvae. (A) Scheme representing enzyme complexes incorporating a functional DLD (E3) subunit: PDHc is the gateway enzyme to the TCA cycle, and AKADHc and BCKADHc are essential enzymes in lysine and BCAA catabolism, respectively. (B) CRISPR/Cas9 construction of dldh?/? zebrafish. Schematic showing where sgRNA targets exon 1 after start codon (ATG); the sgRNA was injected 1 hpf. (C) Sanger confirmation of 5 bp deletion in dldhcri3 zebrafish. (D) Immunoblot of Dldh protein level in 7 dpf WT, dldh+/?, and dldh?/? larvae. (E) ImageJ quantification of Dldh, CS, VDAC, and tubulin protein levels in WT vs. 7 dpf dldh?/? larvae, showing significantly decreased Dldh expression in dldh?/? larvae and increased VDAC levels in dldh+/? and dldh?/?; n = 3; *P < 0.05 and **P < 0.01, unpaired Student?s t test with the Bonferroni correction. Data are shown as mean � SD.

Expression Data

Genes:	cs dldh vdac1
Fish:	AB dldh^cri3/cri3 (AB) dldh^cri3/+ (AB)
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dldh^cri3/cri3 (AB) dldh^cri3/+ (AB)
Observed In:	whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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