FIGURE

Fig. 2

ID

ZDB-FIG-240731-2

Publication

Xie et al., 2024 - Zebrafish myo7aa affects congenital hearing by regulating Rho-GTPase signaling

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Fig. 2

Zebrafish myo7aa gene knockout. (A) Schematic representation of the CRISPR/Cas9 target region in myo7aa gene. Green box represents the 3′UTR and 5′UTR, while the red box indicates exons. (B) Sequence of the CRSIPR/Cas9 target region in the myo7aa gene. Underlined: detection primers; red font: target site sequence; green font: PAM sequence; capital letters: exon sequence; lowercase letters: intron sequences. (C,D) Sequencing peak map, genomic DNA sequence, and amino acid sequence between two independent mutant alleles. Compared with the wild type, the myo7aa line1 mutation had a 114 bp deletion and the line2 mutation had a 106 bp insertion. The sequence of the target site is shown in red. Rectangular boxes indicate deletion or insertion sites in the mutant. *Indicates termination of protein translation. (E) The screening results of the F1 generation adult fish showed the presence of DNA marker, wild type fish, and mutant fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.