Fig. 3

A minor cellular phenotype in calb2b knock-down larvae. A) Brightfield images of control and calb2b knock-down larvae reveal no physical phenotype at 3 days post fertilization (dpf). B) Immunohistochemistry against calretinin at 3 dpf show a significant diminished number of calretinin positives cells. C) Quantification of calretinin positive cells in calb2b knock-down larvae compared to controls. D) Confocal images of dmrt3aRFP in control and calb2b knock-down larvae. E) Quantification of dmrt3aRFP cells in control larvae and calb2b knock-down larvae. F) Confocal images of mnx1RFP in control and calb2b knock-down larvae. G) Quantification of mnx1RFP cells in the control larvae and calb2b knock-down larvae. H) Confocal images of Evx2 positive cells in control and calb2b knock-down larvae. I) Quantification of Evx2 positive cells in control and calb2b knock-down larvae. Scale bar, 100 μm in all images. Statistical differences: >0.05 (ns), ≤0.05 (*), ≤0.01 (**), ≤0.001 (***) and ≤0.0001 (****).