Loss of Mcf2lb leads to the deposition of supernumerary NMs. (A,B) Tg(-8.0claudinB: lynGFP)zf106 marks NMs in WT and mcf2lb mutant embryos at 3 dpf. Note the excess number of deposited trunk NMs in the mcf2lb mutants. Asterisks mark deposited trunk NMs. Full-body images in A were stitched together from four fields of view of individual body segments (head, trunk, tail) using the auto-blend feature in Photoshop. Full-body images in B were stitched together from three fields of view of the head and trunk using the auto-blend feature in Photoshop, and the tail image was manually included. (C) Mean number of NMs in WT (n=8 embryos) and mcf2lb mutant (n=12 embryos) embryos at 3 dpf. (D-E′) DAPI staining of nuclei in deposited NMs in WT (D,D′) and mcf2lb mutants (E,E′). (F) Mean number of cells per NM in WT (n=14 NMs from four embryos) and mcf2lb mutants (n=26 NMs from five embryos). (G) Mean number of cells in the first NM in WT (n=19 NMs) and mcf2lb mutants (n=25 NMs). ***P<0.001 (unpaired two-tailed t-test). n.s., not significant. Error bars are s.d. Scale bars: 100 μm (A,B); 5 μm (D,E).
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