Figure 4

Dram1 can protect against Mm in the absence of Optn and p62. (A-C)dram1 overexpression in single and double mutants of optn and p62. In the experimental workflow, dram1 mRNA was injected at the one cell stage into optn+/+ and -/- embryos (A), p62+/+ and -/- embryos (B), optn+/+p62+/+ and optn-/-p62-/- embryos (C), followed by injection of 200 CFU of Mm into the blood island at 28 hpf and assessment of bacterial burden at 4 dpi (representative images shown). Quantifications show that dram1 could decrease bacterial burden independent of the loss of optn(A), p62(B) or both receptors (C). (D) Overexpression of dram1 in myd88 wild type and mutant background. dram1 mRNA was injected into myd88+/+ and myd88-/- embryos at the one cell stage, followed by infection and bacterial burden assessment as in (A-C) (representative images shown). Quantification showed that dram1 overexpression could not decrease bacterial burden in myd88 mutants. Data are displayed as percentage difference to the control group set at 100% and are accumulated from three (A-C) or two (D) independent infection experiments (each data point representing an individual embryo), indicated with different colors. ns, non-significant, * p<0.05, ***p<0.001.