Neutrophil and macrophage recruitment in viperin-/- fish. (A) Zebrafish lines viperin-/-Tg(mpx:mcherry) and viperin-/-Tg(mpeg:egfp) were generated to track neutrophils and macrophages, respectively. The caudal fin was amputated of these lines and immersed in poly I:C. (B) Analysis of neutrophil recruitment, (C) the recruitment of the macrophages to the site of infection was observed in a time-dependent manner (I-Wt PBS, II-Wt+Poly I:C, III-viperin-/- + PBS, and IV-viperin-/- + Poly I:C). Dynamics of the neutrophils (D), macrophages (E) at the wound site showed time-dependent differences between the WT and viperin-/- fish. Expression analysis of neutrophil marker genes mpx(F) and nox(G) in the adult zebrafish with VHSV infection showed significant upregulation of the marker genes in viperin-/- fish compared to those in WT fish. Gene expression analysis for the marker gene csf1a(H) for macrophages, indicating significant downregulation in viperin-/- fish. Poly I:C, Polyinosinic:polycytidylic acid; hpi, Hours post infection; mpx, myeloid-specific peroxidase; nox, NADPH oxidase; csf1a, colony stimulating factor 1.
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