FIGURE

Fig. 5

ID

ZDB-FIG-231002-100

Publication

Anderson et al., 2023 - Ligament injury in adult zebrafish triggers ECM remodeling and cell dedifferentiation for scar-free regeneration

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Fig. 5

Immune response to ligament transection injury.

UMAPs of single-cell RNA sequencing datasets colored by cluster (a) and injury sample (b). c Dot Plot showing marker gene expression for each immune cluster. Repeated live imaging of neutrophils (d; mpx:mCherry) (n = 5) and macrophages (e; mpeg1.1:eGFP+) (n = 6) at the injury site (arrowheads) during the first week of regeneration color-coded by signal intensity. f Dot Plot demonstrating fn1a expression in macrophage cluster cells split by Sample and Feature Plot for 1 dplt-macrophage enriched gene fn1a. g Representative images of smFISH staining using fn1a (green) and mpeg1.1 (magenta) probes in IOM ligaments from uninjured and 1 dplt samples (n = 3). h Feature Plots for macrophage marker mpeg1.1 and macrophage-enriched gene lgmn. i smFISH for lgmn (yellow) and mpeg1.1 (magenta) probes in IOM ligaments from uninjured and 1 dplt samples (n = 3). Gray dashed lines outline the IOM ligament and inset positions are denoted by the numbers 1-2 (g, i). Scale bars = 500 μm (d, e), 50 μm (g, i).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ NPJ Regen Med