Swimming ability of the sgcd−/− and sgcb−/− larvae. (A) sgcd−/− and wild type sibling larvae, (B) sgcb−/− and wild type sibling larvae at 4 dpf were placed in a 48-well plate and introduced into the DanioVision tracking system. After a few hours of acclimation, the zebrafish larvae movements were recorded for 48 h under a 12:12 light–dark cycle; grey boxes represent the dark periods. The distance moved every 6 min by each larva was recorded and plotted for the entire period considered; each point is the mean distance covered by the number of larvae as indicated, standard deviation was omitted for clarity. (C) Startle test in which 5 dpf zebrafish larvae, upon 20 min of habituation, were subjected to three cycles of 10 min of light, followed by 10 min of dark (grey boxes). The graph reports the mean distance moved every 2 min ± SE of 36 larvae for each genotype. As expected, larvae activity increased during the dark periods, slowing down to a basal level during the light periods. (D) Quantification of the total distance moved during the three dark periods of the startle test by fish of the three genotypes; the average activity value ± SE is also indicated. Statistical analysis was performed using the one-way ANOVA test followed by Dunnett’s multiple comparisons test. ns, p > 0.05; **, p ≤ 0.01.
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