FIGURE

Figure 6

ID

ZDB-FIG-230814-147

Publication

Giardoglou et al., 2023 - Cfdp1 Is Essential for Cardiac Development and Function

Other Figures

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Figure 6

cfdp1 abrogation shows impaired Wnt/β-catenin signaling. (A) Confocal images of cfdp1 morphants show that Wnt/β-catenin reporter activity is diminished compared to the uninjected sibling controls. Max projection of z-stack confocal images of 120 hpf cfdp1-MO embryos. Endothelial cells are labeled with green (Tg(fli1:EGFP)) and Wnt-activated cells are labeled with red (Tg(7xTCF-Xla.Siam:nlsmCherry)); n = 6 in each of three independent experiments. (A’) percentage of phenotypic scoring. AV, atrioventricular valve. B, bulbus arteriosus. Scale bar 150 μm. (B) Confocal images of 120 hpf cfdp1 mutant and wild-type siblings expressing nlsmCherry in Wnt-activated cells and Tg(cmlc2:eGFP) in all cardiomyocytes (Ν = 3, n = 13). Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cfdp1^aa106/aa106; ia5Tg; twu34Tg ia5Tg; y1Tg + MO2-cfdp1
Knockdown Reagent:	MO2-cfdp1
Observed In:	heart Wnt signaling pathway
Stage:	Day 5

Phenotype Detail

Acknowledgments

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