FIGURE

Fig. 3

ID

ZDB-FIG-230717-128

Publication

Nakajima et al., 2023 - Endoderm-derived islet1-expressing cells differentiate into endothelial cells to function as the vascular HSPC niche in zebrafish

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Fig. 3

Figure 3. The Npas4l (Cloche)-Etsrp axis is required for the differentiation of isl1⁺ endothelial progenitors into ECs

(A) TgBAC(isl1:EGFP);Tg(kdrl:mCherry) embryos (25 hpf) injected with control morpholino oligonucleotide (MO) or etsrp MO. The boxed areas are enlarged (bottom). Arrowheads point to ventral isl1:EGFP⁺ cells in the tail.

(B) TgBAC(isl1:EGFP);TgBAC(etsrp:Nls-mCherry) cloche^+/? and cloche^−/− sibling embryos (25 hpf). The boxed areas are enlarged (bottom). isl1:EGFP⁺ cells (arrows) rarely express etsrp:mCherry in cloche mutants.

(C) Quantitative analysis of the data shown in (B). Number of isl1:EGFP/etsrp:mCherry double-positive cells in the tail of cloche^+/? and cloche^−/− sibling embryos. Data are mean ± SD.

(D) TgBAC(isl1:EGFP) cloche^+/? and cloche^−/− sibling embryos (25 hpf) subjected to TUNEL staining. The boxed areas are enlarged on the right. Arrows point to isl1:EGFP/TUNEL double-positive cells.

(E) Quantitative analysis of the data shown in (D). Number of isl1:EGFP/TUNEL double-positive cells in the tail of cloche^+/? and cloche^−/− sibling embryos. Data are mean ± SD.

(F) Time-sequential images of a TgBAC(isl1:dEGFP);npas4lPt(npas4l-p2a-Gal4-VP16);Tg(UAS:RFP) embryo (from 19 hpf). Top, projection view; middle and bottom, single confocal planes of the boxed areas. isl1:dEGFP⁺ cells start to express npas4l:RFP and lose isl1:dEGFP expression (arrows).

Scale bars: 200 μm in (A), 50 μm in (B) and (F), and 20 μm in (D).

See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 58(3), Nakajima, H., Ishikawa, H., Yamamoto, T., Chiba, A., Fukui, H., Sako, K., Fukumoto, M., Mattonet, K., Kwon, H.B., Hui, S.P., Dobreva, G.D., Kikuchi, K., Helker, C.S.M., Stainier, D.Y.R., Mochizuki, N., Endoderm-derived islet1-expressing cells differentiate into endothelial cells to function as the vascular HSPC niche in zebrafish, 224-238.e7, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell