PUBLICATION

Endoderm-derived islet1-expressing cells differentiate into endothelial cells to function as the vascular HSPC niche in zebrafish

Authors
Nakajima, H., Ishikawa, H., Yamamoto, T., Chiba, A., Fukui, H., Sako, K., Fukumoto, M., Mattonet, K., Kwon, H.B., Hui, S.P., Dobreva, G.D., Kikuchi, K., Helker, C.S.M., Stainier, D.Y.R., Mochizuki, N.
ID
ZDB-PUB-230125-6
Date
2023
Source
Developmental Cell   58(3): 224-238.e7 (Journal)
Registered Authors
Dobreva, Gergana, Fukui, Hajime, Helker, Christian, Kikuchi, Kazu, Mochizuki, Naoki, Nakajima, Hiroyuki, Stainier, Didier
Keywords
endothelial specialization, hematopoietic stem and progenitor cell, imaging-based lineage tracing, vascular niche
Datasets
GEO:GSE171822
MeSH Terms
  • Animals
  • Endoderm
  • Endothelial Cells*
  • Endothelium
  • Hematopoietic Stem Cells/physiology
  • Zebrafish*
PubMed
36693371 Full text @ Dev. Cell
Abstract
Endothelial cells (ECs) line blood vessels and serve as a niche for hematopoietic stem and progenitor cells (HSPCs). Recent data point to tissue-specific EC specialization as well as heterogeneity; however, it remains unclear how ECs acquire these properties. Here, by combining live-imaging-based lineage-tracing and single-cell transcriptomics in zebrafish embryos, we identify an unexpected origin for part of the vascular HSPC niche. We find that islet1 (isl1)-expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche. These isl1-expressing cells surprisingly originate from the endoderm and differentiate into ECs in a process dependent on Bmp-Smad signaling and subsequently requiring npas4l (cloche) function. Single-cell RNA sequencing analyses show that isl1-derived ECs express a set of genes that reflect their distinct origin. This study demonstrates that endothelial specialization in the HSPC niche is determined at least in part by the origin of the ECs.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping