FIGURE

Figure 3

ID

ZDB-FIG-230711-12

Publication

Kuil et al., 2023 - Unbiased characterization of the larval zebrafish enteric nervous system at a single cell transcriptomic level

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

One small cluster expresses genes typical for enteric glia in mammalians

(A) Left: Single plane recording showing a HuC/D+phox2bb-neuron. Scale bar represents 10 μm. Right: Maximum projections of HuC/D antibody staining shows that most HuC/D+ cells in the intestine express phox2bb, but also show phox2bb+/HuC/D-cells (progenitors) depicted by the arrows with a circle end, and phox2bb-;HuC/D+ cells (differentiated neurons) depicted by arrows. Scale bar represents 40 μm. Quantification of the relative amount of double and single positive cells, relative to the total number of enteric neurons (HuC/D only, phox2bb only and double-positives combined)(n=9; 5 dpf, error bars show standard deviation).

(B) Dotplot showing selective expression of some known enteric glia and radial glia markers and lack of expression of phox2bb and sox10 in the cluster of enteric glia depicted by the red box.

(C) Maximum projections of live-imaging recordings of 5 dpf photoconverted tg(8.3phox2bb:kaede);tg(her4:GFP) intestines shows phox2bb-;her4+ cell depicted by the arrows, that are in close proximity to, or seem to interact with phox2bb+ neurons (magenta; close interaction between extensions is depicted by the T mark). Scale bar represents 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience