FIGURE

Figure 6

ID

ZDB-FIG-230523-49

Publication

Silva et al., 2023 - In a zebrafish biomedical model of human Allan-Herndon-Dudley syndrome impaired MTH signaling leads to decreased neural cell diversity

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Figure 6

Impaired MTH signaling decreases her2+ neural progenitor cells undergoing mitosis in the spinal cord. (A) Analysis of her2 expression by fluorescent in situ hybridization (green) and mitotic cells (phosphohistone 3 antibody; red) in CTRLMO) and MCT8MO embryos. (B) Box-and-whiskers plot depicting quantification of the number of her2+ mitotic cells (her2+/PH3+) in the spinal cord at 18, 22, and 25hpf. (C) Analysis of her2 expression by fluorescent in situ hybridization (green) and colocalization with apoptotic cell detected using a TUNEL assay (red). (D) Box-and-whiskers plot depicting the quantification of the number of her2+ apoptotic cells in the spinal cord. The images represent a lateral view of the spinal cord between somite 8-12; rostral is to the left in all images; the scale bar represents 50 µm. Colocalization was quantified in the volume of 2 myotomes within this spinal cord region. n=10-15. Statistical significance determined by t-test: two-sample, assuming equal variances. **p<0.01; *** p<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)