FIGURE

Fig. 5

ID

ZDB-FIG-230519-39

Publication

He et al., 2022 - Translational control by maternal Nanog promotes oogenesis and early embryonic development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Depletion of eef1a1l2 ameliorates ER stress and UPR in nanog mutant oocytes. (A-D) RT-qPCR analysis showing decreased expression of hspa5 (A), ddit3 (B), atf6 (C) and ire1a (D) in nanog and eef1a1l2 double-mutant ovaries, compared with nanog mutant ovaries. **P<0.01, ***P<0.001. n=4. (E,F) RT-PCR examination of xbp1 splicing. The ratio of spliced xbp1 (xbp1s) mRNA to unspliced xbp1 (xbp1u) mRNA was increased in nanog mutant ovaries, but restored in nanog and eef1a1l2 double-mutant ovaries. gapdh was used as internal control. The xbp1s/xbp1u ratio in F represents the intensity ratio of the corresponding PCR product bands in E. *P<0.05, **P<0.01. n=3. (G) ER, Golgi and mitochondria structure in WT, nanog mutant and double-mutant stage I oocytes as revealed by transmission electron microscopy. White arrowheads indicate Golgi apparatus, yellow arrowheads indicate mitochondria, red arrowheads indicate lysosomes. Scale bar: 0.5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development