Fig. 4

Depletion of eef1a1l2 rescues impaired oocyte maturation of the nanog mutant. (A) WT, Meef1a1l2, Mnanog and Mnanog,Meef1a1l2 embryos with chorions at 15 mpf. Scale bar: 100 μm. (B,C) Measurement of chorion diameters and oocyte diameters at 15 mpf. *P<0.05, ***P<0.001. NS, no significant difference. n=20. (D) Representative images showing labeling of CGs in WT, nanog and nanog,eef1a1l2 double-mutant eggs fixed at 10 mpa. F-actin was stained using phalloidin to show the outline of the embryo. n=15. Scale bar: 100 μm. (E) Appearance of ovaries (outlined) dissected from WT, eef1a1l2^−/−, nanog^−/− and nanog^−/−,eef1a1l2^−/− females. Scale bar: 1 mm. (F) GSI of WT, eef1a1l2^−/−, nanog^−/− and nanog^−/−,eef1a1l2^−/− females. n=3. *P<0.05, ***P<0.001. NS, no significant difference. (G) Morphology of stage V follicles from WT, eef1a1l2^−/−, nanog^−/− and nanog^−/−,eef1a1l2^−/−. Insets show enlarged regions of the yolk. Scale bar: 100 μm. (H) SDS-PAGE and Coomassie staining of major yolk proteins of stage III and stage V follicles. The higher and lower molecular weight yolk proteins (HYP and LYP) are indicated by red arrows. HYP/LYP ratios (shown above) were calculated to represent yolk protein cleavage levels. (I) Morphology of stage IV follicles dissected from WT, eef1a1l2^−/−, nanog^−/− and nanog^−/−,eef1a1l2^−/− ovaries with or without DHP (1 μg/ml) incubation for 2 h. Scale bar: 1 mm. (J) Comparison of the GVBD percentage in WT, eef1a1l2^−/−, nanog^−/− and nanog^−/−,eef1a1l2^−/− follicles. **P<0.01, ***P<0.001. NS, no significant difference. n=4.