FIGURE

Fig. 5

ID

ZDB-FIG-230324-5

Publication

Xie et al., 2022 - Cilia regulate meiotic recombination in zebrafish

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Fig. 5

Ift88 deficiency results in ciliogenesis defects and apoptosis of spermatocytes. (A) Schematic workflow showing the strategy of generating Tg(hsp70l:ift88-egfp) transgene to rescue ovl(ift88) mutants. (B) Statistical analysis of the percentage of ciliated spermatocytes in heat-shocked and non-heat-shocked ift88;Tg(hsp70l:ift88-egfp) transgenic fish. (C) Confocal images showing cilia and synaptonemal complexes labelled with anti-acetylated tubulin (green) and anti-SYCP3 (red) antibodies on primary spermatocytes from different fish as indicated. (D and E) Confocal images showing apoptotic cells stained by TUNEL assay (D) and the statistical results (E) of TUNEL-positive cells in heat-shocked and non-heat-shocked fish as indicated. **P < 0.01. wt, wild type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.