ZFIN Image: Xie et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Ift88 deficiency results in ciliogenesis defects and apoptosis of spermatocytes. (A) Schematic workflow showing the strategy of generating Tg(hsp70l:ift88-egfp) transgene to rescue ovl(ift88) mutants. (B) Statistical analysis of the percentage of ciliated spermatocytes in heat-shocked and non-heat-shocked ift88;Tg(hsp70l:ift88-egfp) transgenic fish. (C) Confocal images showing cilia and synaptonemal complexes labelled with anti-acetylated tubulin (green) and anti-SYCP3 (red) antibodies on primary spermatocytes from different fish as indicated. (D and E) Confocal images showing apoptotic cells stained by TUNEL assay (D) and the statistical results (E) of TUNEL-positive cells in heat-shocked and non-heat-shocked fish as indicated. **P < 0.01. wt, wild type.

Acknowledgments

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