FIGURE

Figure 1

ID

ZDB-FIG-230321-11

Publication

Xiao et al., 2023 - Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish

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Figure 1

Dopey2+ cells exhibit higher stemness capacity

(A) Fluorescence-activated cell sorted Dopey2⁺ and Dopey2^- NSCs/neural progenitor cells were seed in semi-solid NSC medium. After 7 days, Dopey2⁺ NSCs/neural progenitor cells formed larger clones. Scale bar = 1 mm.

(B) In NCFC assay, neurospheres cultured as monolayer for differentiation were multi-labeled immunostained for DAPI/Mbp/GFAP and DAPI/Tuj/MAP2. Scale bar = 50 μm.

(D) Neurospheres were measured and grouped. Tripotential clone: differentiating into three types of neural cells; bipotential clone: differentiating into either two cell types; monopotential clone: differentiating into one cell type.

(E) Stemness calculated by dividing the number of forming tripotential clones by total cells. Student’s t test: ∗∗∗p < 0.001 indicates a significant increase when Dopey2⁺ group compared with Dopey2^- group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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