ZFIN Image: Xiao et al., 2023, Figure 1

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Figure Caption

Figure 1

Dopey2+ cells exhibit higher stemness capacity

(A) Fluorescence-activated cell sorted Dopey2⁺ and Dopey2^- NSCs/neural progenitor cells were seed in semi-solid NSC medium. After 7 days, Dopey2⁺ NSCs/neural progenitor cells formed larger clones. Scale bar = 1 mm.

(B) In NCFC assay, neurospheres cultured as monolayer for differentiation were multi-labeled immunostained for DAPI/Mbp/GFAP and DAPI/Tuj/MAP2. Scale bar = 50 μm.

(C) Colony number of Dopey2⁺ and Dopey2^- neuroshperes (means ± SEM; n = 3).

(D) Neurospheres were measured and grouped. Tripotential clone: differentiating into three types of neural cells; bipotential clone: differentiating into either two cell types; monopotential clone: differentiating into one cell type.

(E) Stemness calculated by dividing the number of forming tripotential clones by total cells. Student’s t test: ∗∗∗p < 0.001 indicates a significant increase when Dopey2⁺ group compared with Dopey2^- group.

Acknowledgments

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