FIGURE

Fig. 3

ID

ZDB-FIG-230228-431

Publication

Mattis et al., 2023 - Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Partial loss of RREB1 reduces cellular insulin content in mature beta cells. (a) RREB1 gene expression (normalised to the housekeeping genes TBP and PPIA and expressed as % of siNT) in siNT control and siRREB1 knockdown EndoC-?H1 cells (n=3). (b) Representative western blot of RREB1 (250 kDa) and tubulin (50 kDa) in siNT and siRREB1 EndoC-?H1 cells. (c) Quantification of RREB1 protein expression following siRNA knockdown in EndoC-?H1 cells (n=6). (d) INS gene expression (normalised to the housekeeping genes TBP and PPIA and expressed as % of siNT) in siNT and siRREB1 EndoC-?H1 cells (n=5). (e) Cellular insulin content (pg/cell) (n=5) and (f) glucose-stimulated insulin secretion (pg/ml) (n=6; n=5 for siRREB1 16.7 mmol/l glucose) measured in siNT and siRREB1 EndoC-?H1 cells. Data are presented as means�SEM. *p<0.05, **p<0.01 (unpaired t test)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Diabetologia