FIGURE

Figure 2

ID

ZDB-FIG-230211-130

Publication

Morgan et al., 2023 - ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

ahctf1 heterozygosity disrupts the density of nuclear pore complexes and reduces nuclear volume in dox-treated TO(kras^G12V)^T/+ hepatocytes.

(a) Representative Airyscan imaging of liver sections stained with mAb414 (white) marking FG-NUPs, Hoechst 33342 (cyan) marking DNA, and rhodamine phalloidin (magenta) marking the F-actin cytoskeleton in non-TO(kras^G12V)-expressing cells and EGFP-Kras^G12V (magenta) marking the cell membrane in dox-treated TO(kras^G12V)-expressing cells of the indicated ahctf1 and TO(kras^G12V) genotypes. Arrows in right-hand panel point to mAb414/FG-nucleoporin staining in the cytoplasm. Scale bar 2 µm. (b) Quantification of mean nuclear/cytoplasmic fluorescence intensity of mAb414 staining after 3D segmentation and morphological filtering of nuclear and cytoplasmic areas (n > 18). (c) Representative Airyscan images of mAb414 staining at the nuclear surface of sections of the indicated ahctf1 and TO(kras^G12V) genotype. Scale bar 1 µm. (d) Quantification of nuclear pore density (n ≥ 25). (e) Quantification of nuclear volume (n ≥ 25). Data are expressed as mean ± standard error of the mean (SEM). Significance was assessed using a one-way analysis of variation (ANOVA) with Tukey’s multiple comparisons test.

Expression Data

Antibody:	Ab1-nucleoporin
Fish:	gz32Tg/+ ahctf1^ti262c/+; gz32Tg/+
Condition:	chemical treatment by environment: doxycycline
Anatomical Term:	hepatocyte nuclear pore
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg/+ ahctf1^ti262c/+; gz32Tg/+
Condition:	chemical treatment by environment: doxycycline
Observed In:	hepatocyte nuclear pore
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife

Figure 2

Figure 2

ahctf1 heterozygosity disrupts the density of nuclear pore complexes and reduces nuclear volume in dox-treated TO(krasG12V)T/+ hepatocytes.

ahctf1 heterozygosity disrupts the density of nuclear pore complexes and reduces nuclear volume in dox-treated TO(kras^G12V)^T/+ hepatocytes.