FIGURE

Fig. 2

ID

ZDB-FIG-230202-42

Publication

Wilms et al., 2021 - Glutaredoxin 2 promotes SP-1-dependent CSPG4 transcription and migration of wound healing NG2 glia and glioma cells: Enzymatic Taoism

Other Figures

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Fig. 2

Glutaredoxin 2c increases migratory capacity of mouse NG2⁺ glia. A) NG2⁺ cells were seeded in a Boyden chamber and incubated with 2 μM Grx2cwt or Grx2cC37S or transfected either with control siRNA or Grx2siRNA. Transmigrated cells were counted. B) Two-photon-microscopy of dorsally migrating oligodendrocyte progenitor cells in Tg(olig2-GFP) zebrafish at 3 dpf with and without morpholino-induced Grx2 knock-down. C) Migrated cells shown in b) were counted in zebrafish 3 dpf comparing Grx2 knock-down or Grx2 overexpression with respective controls. D) Quantified gap closure after scratch assay at indicated time points by NG2⁺ cells treated with 2 μM recombinant Grx2c or not. E) Immunocytochemistry of control and Grx2c-treated NG2⁺ cells after scratch assay at indicated time points. *: p < 0.05; **: p < 0.01; ***: p < 0.001 (Student's t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ki4Tg (AB) vu12Tg + MO1-glrx2
Knockdown Reagent:	MO1-glrx2
Observed In:	glioblast (sensu Vertebrata) cell migration
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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