Fig. 1
- ID
- ZDB-FIG-230126-17
- Publication
- do Amaral et al., 2022 - The perinuclear region concentrates disordered proteins with predicted phase separation distributed in a 3D network of cytoskeletal filaments and organelles
- Other Figures
- All Figure Page
- Back to All Figure Page
An intricated network of cytoskeletal filaments and several organelles are found in the nuclear space of eukaryotic cells. (A and B) COS-7 cells were extracted and analyzed under transmission electron microscopy. Note the presence of a dense network (in A and B) of cytoskeletal filaments (Fi) linked to the nucleus (Nu). In the higher magnification (B) it is possible to see several protein aggregates (Ag) attached to the cytoskeletal network. Scale bars in A and B = 500 ηm. (C–G) 2-month-old mouse cardiac tissues were processed for transmission electron microscopy and images show mitochondria (Mt), Golgi apparatus (G), vesicles (Ve), myofibers (Mf), and cytoskeletal filaments (Fi) in proximity with the nucleus (Nu). Arrow in (C) shows cytoskeletal filaments in the perinuclear space, arrow in (D) shows mitochondria in close contact with the outer nuclear membrane, and arrow in (G) points to the Z-disk of a myofibril in the vicinity of the nucleus. Scale bars in C-G = 2 μm. N = 3 independent experiments. |
Reprinted from Biochimica et biophysica acta. Molecular cell research, 1869, do Amaral, M.J., de Andrade Rosa, I., Andrade, S.A., Fang, X., Andrade, L.R., Costa, M.L., Mermelstein, C., The perinuclear region concentrates disordered proteins with predicted phase separation distributed in a 3D network of cytoskeletal filaments and organelles, 119161, Copyright (2022) with permission from Elsevier. Full text @ BBA Molecular Cell Research