Fig 3

A) Survival of three mutant zebrafish lines, mutant94, mutant450, and mutant14, is compromised in pneumococcal infection compared to wild type (AB) fish and other mutant lines (mutant445 and mutant12). In the experiment, mutant and AB embryos were infected with 35–440 cfu of S. pneumoniae at 2 dpf. The graphs represent the results from three (mutant94, mutant450, mutant14, AB) or one survival experiment (mutant445 and mutant12) (n = 24–48 in each). B) Bacterial burden in mutant94 larvae is elevated after pneumococcal challenge compared to AB and other hypersusceptible mutant lines. Embryos were infected with 505 cfu of S. pneumoniae at 2 dpf and bacterial counts were determined at 18 hpi. The circles represent bacterial counts in a single larva from one experiment (n = 8). Median bacterial count is depicted with a line. The statistical comparison of difference was calculated with log-rank (Mantel-Cox) test in A, and with Kruskal-Wallis test with Dunn’s multiple comparisons test in B. cfu = colony forming units.