FIGURE

Figure 5.

ID

ZDB-FIG-230115-44

Publication

Bearce et al., 2022 - Urotensin II-related peptides, Urp1 and Urp2, control zebrafish spine morphology

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Figure 5.

Reissner fiber (RF) breakdown in cfap298^tm304 mutants but not urotensin-deficient mutants.

(A–F) Grayscale maximal intensity projection of Sspo-GFP localization in the central canal in 28 hpf embryos (A–C) and 12 dpf adolescents (D–F). RF is denoted by arrow heads in D and F. Arrows point to structures along the central canal that become GFP-positive in cfap298^tm304 and uts2r3^b1436 mutants. Scale bar: 10 µm. (G) Schematic of temperature shift experiment in which cfap298^tm304 mutants are initially raised at permissive temperatures before being shifted to restrictive temperatures at 6 dpf, then imaged at 12 dpf. (H–I) Lateral views of cfap298^tm304 (H) and uts2r3^b1436 (I) mutants at 12 dpf when Sspo-GFP imaging took place. The white box in H shows the location imaged in D–F.

Expression Data

Gene:	EGFP
Fish:	sspo^ut24Tg cfap298^tm304/tm304; sspo^ut24Tg uts2r3^b1436/b1436; sspo^ut24Tg (AB)
Anatomical Terms:	central canal Reissner's fiber
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cfap298^tm304/tm304; sspo^ut24Tg uts2r3^b1436/b1436; sspo^ut24Tg (AB)
Observed In:	central canal Reissner's fiber
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

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Figure 5.

Figure 5.

Reissner fiber (RF) breakdown in cfap298tm304 mutants but not urotensin-deficient mutants.

Reissner fiber (RF) breakdown in cfap298^tm304 mutants but not urotensin-deficient mutants.