FIGURE

Figure 1

ID

ZDB-FIG-221226-324

Publication

Weeks et al., 2022 - Embryonic alcohol exposure disrupts the ubiquitin-proteasome system

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Figure 1

RNA-Seq identifies the ubiquitin proteasome system as a target of embryonic alcohol exposure.

(A) Schematic of RNA-Seq performed on 7 dpf whole-larval extracts following 0% or 1% EtOH exposure (12 hpf–5 dpf). (B) Heatmap of significantly dysregulated genes (P_adj < 0.05; n = 1,603). (C) GOrilla GSEA identifies GO components enriched in the dysregulated gene set. (D) Heatmap of genes involved in proteasome-mediated, ubiquitin-dependent protein catabolism. Following EAE, these genes were significantly upregulated relative to controls. (E) ISH for psmb1 following 0% or 1% EtOH exposure (12–96 hpf). At 96 hpf, psmb1 expression is increased in the liver (black arrow) and intestine (white star). Scale bars: 100 μm. (F) Overview of the protocol for treatment and isolation of GFP⁺ hepatocytes via FACS sorting at 120 hpf. (G) ef1α-normalized qPCR of proteasome-related genes in GFP⁺ hepatocytes sorted by FACS (*P ≤ 0.05, **P ≤ 0.01, 2-sided t test; n = 5 per column). Data represent mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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