FIGURE

Figure 4

ID

ZDB-FIG-221226-318

Publication

Bandla et al., 2022 - A New Transgenic Tool to Study the Ret Signaling Pathway in the Enteric Nervous System

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

The majority of nitrergic and serotonergic neurons are GFP⁺. ret:GFP is expressed in the majority of nitrergic (A?A?) and serotonergic (B?B?) neurons (white). Insets in (A?,B?) show close-up of the boxed area, GFP, nNOS or 5-HT, and overlay from left to right. Schematic of boxed area of larval schematic (C) indicates the four gut sub-regions analyzed (D). (E) Quantification of GFP and nNOS colocalization in four gut regions as shown in (D). Bar graph shows % GFP⁺/nNOS⁺ out of total nNOS⁺ cells (yellow), and % GFP^?/nNOS⁺ out of total nNOS⁺ cells (magenta). Error bars show �standard error of the mean (2 experiments, 23 larvae). (F) Quantification of GFP and 5-HT colocalization in four gut regions shown in D. Bar graph shows % GFP⁺/5-HT⁺ out of total 5-HT⁺ cells (yellow), and % GFP^?/5-HT⁺ out of total 5-HT⁺ cells (magenta). Using 2-way ANOVA, we did not find significant differences between the cell population of the different gut regions. Error bars show �standard error of the mean (2 experiments, 22 larvae). (A?A?,B?B?): maximum projections of dissected guts. Scale bar = 100 �m.

Expression Data

Gene:	EGFP
Fish:	b1331Tg
Anatomical Terms:	enteric nervous system nitrergic neuron enteric nervous system serotonergic neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.