FIGURE

Figure 3

ID

ZDB-FIG-221226-317

Publication

Bandla et al., 2022 - A New Transgenic Tool to Study the Ret Signaling Pathway in the Enteric Nervous System

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

The majority of GFP⁺ cells are ENS neurons at later stages of ENS development. At 5 (A?A?) and 7 (B?B?) days post fertilization (dpf) GFP⁺ ENS neurons (white) are found along the whole length of the gut. A smaller fraction are GFP⁺ non-neuronal cells (green) or GFP^? ENS neurons (magenta). Insets in (A?,B?) show the close-up of the boxed area, GFP, Elavl, and overlay from left to right. Schematic of boxed area of larval schematic (C) indicates the four gut sub-regions analyzed (D). Quantification of GFP and Elavl colocalization at 5 dpf (E) and 7 dpf (F) in four gut regions shown in (D). Bar graph shows % GFP⁺/Elavl⁺ out of total number of cells quantified [GFP⁺/Elavl⁺, GFP⁺/Elavl^?, and GFP^?/Elavl⁺], (yellow), % GFP⁺/Elavl^? out of total cells (green), and % GFP^?/Elavl⁺ out of total cells (magenta). Using 2-way ANOVA, we did not find significant differences between the cell populations of the different gut regions except where indicated (** p ? 0.01). Error bars show �standard error of the mean (5 dpf: 2 experiments, 19 larvae; 7 dpf: 2 experiments, 15 larvae). (A?A?,B?B?): maximum projections of dissected guts at stage indicated. Scale bar = 100 �m.

Expression Data

Gene:	EGFP
Fish:	b1331Tg
Anatomical Term:	enteric neuron
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.