FIGURE

Fig. 3

ID

ZDB-FIG-221223-3

Publication

Lei et al., 2021 - Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafish

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Fig. 3

gnai2a is selectively required for lymphatic development in zebrafish embryos

(A) Relative mRNA expression of different G-protein-coupled receptors in HDLECs.

(B) Relative mRNA expression of PROX1 in HDLECs upon transfection with ctr-siRNA (control), GNA11-siRNA, GNA13-siRNA, and GNAI2-siRNA.

(D) WISH of gnai2a gene expression (upper) and gnai2b gene expression (below) at 26 hpf in zebrafish embryos. The white arrowhead indicates the PCV area.

(E) Confocal images showing TD formation in Tg(fli1a:EGFP) injected with 4 ng control MO, 4 ng gnai2a MO, 4 ng gnai11a MO, 4 ng gnai11b MO, 4 ng gna13a MO, and 4 ng gna13b MO at 5 dpf. Scale bars: 100 μm.

(F) Percentage of somites lacking TD formation. For each group, 30 embryos were quantified, and 6 somites/embryo were used for quantification. In (A), (B), and (F), values represent means ± SEMs. ∗p ≤ 0.001; ns, not significant in Student's t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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