ZFIN Image: Lei et al., 2021, Fig. 3

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Figure Caption

Fig. 3

gnai2a is selectively required for lymphatic development in zebrafish embryos

(A) Relative mRNA expression of different G-protein-coupled receptors in HDLECs.

(B) Relative mRNA expression of PROX1 in HDLECs upon transfection with ctr-siRNA (control), GNA11-siRNA, GNA13-siRNA, and GNAI2-siRNA.

(C) Western blot analysis of PROX1 and GNAI2 expression upon GNAI2 knockdown in HDLECs.

(D) WISH of gnai2a gene expression (upper) and gnai2b gene expression (below) at 26 hpf in zebrafish embryos. The white arrowhead indicates the PCV area.

(E) Confocal images showing TD formation in Tg(fli1a:EGFP) injected with 4 ng control MO, 4 ng gnai2a MO, 4 ng gnai11a MO, 4 ng gnai11b MO, 4 ng gna13a MO, and 4 ng gna13b MO at 5 dpf. Scale bars: 100 μm.

(F) Percentage of somites lacking TD formation. For each group, 30 embryos were quantified, and 6 somites/embryo were used for quantification. In (A), (B), and (F), values represent means ± SEMs. ∗p ≤ 0.001; ns, not significant in Student's t test.

Acknowledgments

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