Figure 8.
- ID
- ZDB-FIG-221211-316
- Publication
- Nelson et al., 2022 - The developmental progression of eight opsin spectral signals recorded from the zebrafish retinal cone layer is altered by the timing and cell type expression of thyroxin receptor β2 (trβ2) gain-of-function transgenes
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Adult cone PIII spectral properties in mpv17−/− and gnat2:mYFP-2A-trβ2;mpv17−/− retinas. A, Cone signals in an mpv17−/− control eyecup are evoked by all stimulus wavelengths and all but the dimmest irradiances (black traces). The largest amplitudes occur at 490 nm. B, Cone signals from a gnat2:trβ2 eyecup are stimulated by all wavelengths and irradiances. Greatest amplitudes occur at 650, 570, and 490 nm, with a larger amplitude spectral pattern at 650 and 570 nm than in the control (up arrows). A, B, Adults are 8–18 months. Eyecups are perfused with medium containing 10 mm aspartate to isolate retinal cone signals. Stimulus irradiances [log(quanta·μm−2·s−1)] appear in the legends to the right of irradiance-response waveform stacks. C, Voltage distributions of maximal trough-to-peak amplitudes found in spectral datasets. D, Cone-signal latencies from stimulus onset to the minimum in the ON trough. E, Cone-signal latency from stimulus offset to the OFF peak. C, D, E, The n.s. labels on all distributions indicate that that mpv17−/− controls did not significantly differ from gnat2:trβ2 transgenics in waveform characteristics (t test and p-values given in text). Peak amplitudes (dataset Vmax), trough (PIII ON trough), and peak (PIII OFF peak) latencies are measured on the mean waveforms from each 70-stimulus spectral dataset. |