Figure 5.

Spectral models of cumulative data from 6-dpf embryonic crx:mYFP-2A-tr?2 and wild-type eyes. A, WT irradiance-response datapoints, SEs, and optimal model (#111) curves as fit to 1858 spectral response amplitudes combined from 28 normalized datasets taken from 10 eyes (Fig. 1A, Eq. 1 algorithm); 370 nm, n = 23?28; 490 nm, n = 27?28; 570 nm; n = 23?28; 650 nm, n = 23?55. B, crx:tr?2 irradiance-response datapoints and optimal model (#202) curves fit to 1860 spectral points combined from 29 datasets taken from 11 eyes. Individual points: 370 nm, n = 22?28; 490 nm, n = 23?28; 570 nm, n = 27?28; 650 nm, n = 24?55. The crx:tr?2 transgene moves curves and points along the irradiance axis as compared with WT. C, In WT, six cone opsin types (gray bars) were detected by the optimal model. In crx:tr?2, four cone opsin signals were detected (magenta bars). Cone saturation amplitudes [Vm_i values (Eq. 1; Fig. 2A) ± SE] are fractions of dataset maximal amplitudes. Asterisks denote significance of differences between WT and crx:tr?2 (one-sample or two-sample t tests; n.s., not significant; GraphPad Prism convention). Vu358 (UV, one-sample test): t₍₁₈₂₂₎ = 17.8, p = 1.2 × 10^?65; Vb415 (B1): t₍₃₆₄₇₎ = 0.958, p = 0.338; Vb440 (B2): t₍₁₈₂₂₎ = 4.27, p = 2.0 × 10^?5; Vg460 (G1): t₍₃₆₄₇₎ = 1.87, p =0.062; Vg500 (G4, one-sample test): t₍₁₈₂₂₎ = 3.33, p = 8.8 × 10^?4; Vr556 (R2): t₍₃₆₄₇₎ = 0.318, p = 0.751; Vr575 (R1): t₍₁₈₂₅₎ = 2.72, p = 0.0067. D, Spectral peaks shift to longer wavelengths for crx:tr?2 (magenta) as compared with WT (black). Spectral curves are the modeled amplitudes that would be evoked by three different irradiances of constant quantal stimulation across the spectrum [3.4, 4.0, and 4.6 log(quanta·?m^?2·s^?1)]. Spectral shapes differ with stimulus brightness. A, B, The log(k_R1R2) values are modeled R1-cone and R2-cone semi-saturation irradiances in log(quanta·?m^?2·s^?1). A?D, 20 mm aspartate medium.