FIGURE

Fig. 4

ID

ZDB-FIG-221123-28

Publication

Rocha et al., 2021 - Zebrafish Cdx4 regulates neural crest cell specification and migratory behaviors in the posterior body

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Fig. 4

cdx4 mutants show impaired segmental migration of trunk NC cells. A-C. Lateral view of 24 hpf WT embryo labeled with anti-Sox10 (A, NC cells, magenta) and anti-Myosin Heavy Chain (B, somites, green) antibodies reveal segmental migration pathways of trunk NC cells, scale bar = 100 μm. A’-C’. High magnification images of boxed area from A-C (arrows = individual segmental chains), scale bar = 50 μm. D-F. Lateral view of 24 hpf cdx4^ch107−/- embryo labeled with anti-Sox10 (D, NC cells, magenta) and anti-Myosin Heavy Chain (E, somites, green) antibodies reveal defects in trunk NC cell migration. Scale bar = 100 μm. D’-F’. High magnification images of boxed area from D-F, scale bar = 50 μm. G. Schematic representing approach for mapping the position of trunk NC cells relative to the adjacent somite; analysis was performed at the level of somites 5–10. H, I. Plots of migrating trunk NC cells relative to the adjacent somite in WT embryos (H) and cdx4^ch107−/- embryos (I). Data from 18 total segments were superimposed on each plot.

Expression Data

Antibodies:	Ab-A4.1025 Ab3-sox10
Fish:	cdx4^ch107/ch107
Anatomical Terms:	somite trunk neural crest cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdx4^ch107/ch107
Observed In:	migratory trunk neural crest neural crest cell migration trunk neural crest cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 480, Rocha, M., Kushkowski, E., Schnirman, R., Booth, C., Singh, N., Beadell, A., Prince, V.E., Zebrafish Cdx4 regulates neural crest cell specification and migratory behaviors in the posterior body, 25-38, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.