Fig. 4

Fig. 4 cdx4 mutants show impaired segmental migration of trunk NC cells. A-C. Lateral view of 24 hpf WT embryo labeled with anti-Sox10 (A, NC cells, magenta) and anti-Myosin Heavy Chain (B, somites, green) antibodies reveal segmental migration pathways of trunk NC cells, scale bar ​= ​100 ​μm. A’-C’. High magnification images of boxed area from A-C (arrows ​= ​individual segmental chains), scale bar ​= ​50 ​μm. D-F. Lateral view of 24 hpf cdx4^ch107−/- embryo labeled with anti-Sox10 (D, NC cells, magenta) and anti-Myosin Heavy Chain (E, somites, green) antibodies reveal defects in trunk NC cell migration. Scale bar ​= ​100 ​μm. D’-F’. High magnification images of boxed area from D-F, scale bar ​= ​50 ​μm. G. Schematic representing approach for mapping the position of trunk NC cells relative to the adjacent somite; analysis was performed at the level of somites 5–10. H, I. Plots of migrating trunk NC cells relative to the adjacent somite in WT embryos (H) and cdx4^ch107−/- embryos (I). Data from 18 total segments were superimposed on each plot.