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Salvage pathway genes contribute to vascular integrity. The fcsk gene is expressed ubiquitously in the head of wildtype 48 hpf embryos (A) compared to the mild background staining in the wildtype control embryos using a sense probe (B). The fpgt gene was expressed ubiquitously in the head of wildtype 48 hpf embryos (C) compared to the mild background staining in the wildtype control embryos using a sense probe (D). The recorded cerebral hemorrhaging rates of embryo clutches derived from gmds heterozygous matings and control wildtype crosses with or without fcsk morpholino (E), showing that the fcsk morpholino increase hemorrhage rates. (F), results of rescue experiments using GDP-fucose and L-fucose, demonstrating that both conditions can rescue hemorrhage rate. Statistical significance was calculated using a chi-squared test with Yate's correction. Error bars represent standard error of the mean hemorrhage rate from three independent experiments.
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