Fig. 2

Fig. 2 Salvage pathway genes contribute to vascular integrity. The ​fcsk ​gene is expressed ubiquitously in the head of wildtype 48 hpf embryos (A) compared to the mild background staining in the wildtype control embryos using a sense probe (B). The ​fpgt ​gene was expressed ubiquitously in the head of wildtype 48 hpf embryos (C) compared to the mild background staining in the wildtype control embryos using a sense probe (D). The recorded cerebral hemorrhaging rates of embryo clutches derived from ​gmds ​heterozygous matings and control wildtype crosses with or without ​fcsk ​morpholino (E), showing that the ​fcsk ​morpholino increase hemorrhage rates. (F), results of rescue experiments using GDP-fucose and L-fucose, demonstrating that both conditions can rescue hemorrhage rate. Statistical significance was calculated using a chi-squared test with Yate's correction. Error bars represent standard error of the mean hemorrhage rate from three independent experiments.